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In vitro BP incorporation was performed at 37 C for 1 h, and then, the samples were diluted with sample loading buffer (60 mM Tris-Cl (pH 6.8), 10% glycerol, 2% SDS, 1% -mercaptoethanol (ME), 0.01% bromophenol blue) and boiled at 100 C for 10 min

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Western blot analysis Total protein of cartilage tissues were extracted using a radio immunoprecipitation assay (RIPA) buffer containing sodium pyrophosphate and protease inhibitors

Diarrhea is a documented side effect, and for some, it can be severe enough to cause irritation in the rectal canal

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