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To determine whether ROS (known as secondary messengers in cellular signaling) participate in LPA signaling, cells were treated with the NADPH oxidase inhibitor, diphenyleneiodonium (DPI), and antioxidants such as NAC, EUK-134, and curcumin

tritici (Ggt) on the roots

Cells were analysed by flow cytometry using a BD LSR II instrument

The process involves feeding empty ampoules into ultrasonic washing unit from where these ampoules are passed through sterilization tunnel, filling station, and sealing unit etc