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Overall, across the two repeat experiments for Cagri and CagriBB bound to the CTR, the conformational equilibrium between the bypass vs CT-like state was similar, suggesting that the salt bridge/ionic lock is sufficient to preferentially stabilize the bypass conformation at CTR (Supplementary Fig

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According to the instructions of the Annexin V-FITC Apoptosis Detection Kit (Beyotime, C1062), 5 L of Annexin V-FITC reagent was added and gently mixed, followed by the addition of 10 L of propidium iodide staining solution, which was also gently mixed

No strategy guarantees zero regain, but tapering significantly reduces the risk of rapid rebound

Purity verification: All research-grade peptides should have documented purity analysis