Photocoagulation surgery
[cited 2024 June 13];7
Suitable for daily use, morning and night
the freeze-drying process comprises the following steps, preparing a liquid and filling at the temperature of 20-30 , putting a sample into a freeze dryer, quickly reducing the temperature of a plate layer to-40 , preserving heat for 1h, when the temperature of a partition plate is-10 +/-2 , preserving heat for 1h, quickly reducing the temperature of the plate layer to-40 within 1h, preserving heat for 3h, transferring to a cold trap at-50 , vacuumizing to below 10Pa, firstly preserving the temperature of the plate layer at-5 for 3h, then heating the plate layer to 10 within 3h, when a product is at 0 , starting to increase the temperature of the plate layer to 40 , and when the temperature of the product is increased to 30 , preserving heat for 3 h

Measurement of reductive GSH Reduced GSH assay (Solarbio, Beijing, China) has been used to determine the iron content in cells based on the yellow 2-nitro-5-mercaptobenzoic acid produced by glutathione and 5,5-dithiobis-2-nitrobenzoic acid (DTNB), which was detected at a wavelength of 412 nm