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Glutathione is an antioxidant produced in cells
Protein concentration was estimated according to 2.5.1 Glutathione Reduced glutathione was measured fluorometrically using the method of o -phthaldialdehyde (1 mg/ml) for 15 min
The enzymes involved are: The biosynthesis of glutatione in cells is accomplished by six enzymes found on the plasma membrane and in the cytosol from the precursor amino acids Glu, Cys, and Gly
Detection would require a specific peptide mass spectrometry assay developed for that purpose, which is not commercially available for retatrutide and is not part of any standard testing program
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