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Strong interest in TB-500s effect on wound healing stems from a 2005 Phase I clinical trial, which found that the peptide plays a key role in dermal- and corneal-wound healing and could support healing in chronic cutaneous wounds [11]

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Then, isolated RNA was reverse transcribed into cDNA using the Primescript RT Master Mix (RR036A, TaKaRa) with incubation at 37 C for 15 min and, then, at 85 C for 5 s, subsequently terminated by incubation at 4 C forever using a commercial Thermal Cycler (C1000, Bio-Rad)

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