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Both can become depleted through poor diet and aging
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For antibody staining, cells were incubated with Fc block (Human TruStain FcX, BioLegend, 422302, 1:20) for 15 min, then incubated with antibodies in ice-cold 10% FBS, 1 PBS, 2 mM EDTA for 20 min at 4 C and then washed with 1 PBS and resuspended in 10% FBS, 1 PBS and 2 mM EDTA before analysis using a BD LSR Fortessa X-20 flow cytometer with FACS DIVA software (v.9.0.1)
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