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doi: 10.1038/mp.2016.3 185 UpthegroveRManzanares-TesonNBarnesNM

Suited to in-vitro or preclinical work requiring lyophilized peptides reconstituted under aseptic technique with proper cold storage

Ice-crystal formation during each freeze-thaw cycle inflicts mechanical damage on peptide chains, and pre-splitting eliminates the cumulative loss that comes from thawing one vial multiple times

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Treated tendons showed accelerated recovery with increased load to failure, meaning they could withstand greater force before breaking