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Endogenously present in human plasma, its levels decline with age
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The GSH/GSSG ratio can be measured in specific organelles using redox-sensitive green fluorescent proteins (roGFPs), particularly those fused to the GSH-specific Grx1 protein 7
The supernatant was then analyzed by HPLC with a Rainin Econosphere ODS C18 column and an acetonitrile/water mobile phase containing 0.1% trifluoroacetic acid
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