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Encapsulation efficiency was measured using ICP-MS and revealed excellent encapsulation efficiency of DDA-GSH-AuNCs into PLGA matrix (93.09 1.71%), owing to the hydrophobic character of the nanoclusters that prevents its partition into the external polar phase (ethanol) and rather get encapsulated in the hydrophobic PLGA matrix

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Beads were initially equilibrated in 20 mM Tris-HCl pH 8.0, 300 mM NaCl binding buffer, followed by incubation with 50 M His-tagged Syn constructs (Syn WT or Syn 1103 ) for 45 min at 4 C with gentle rotation

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Normal erectile function involves 3 synergistic and simultaneous processes : 1) neurologically mediated increase in penile arterial inflow, 2) relaxation of cavernosal smooth muscle and 3) restriction of venous outflow from the penis

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