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Generation of ROS, including hydrogen peroxide, is one of the early events common to many of these pathways, and is referred to as the oxidative burst stage (Orozco-Cardenas and Ryan 1999)

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Changing the amount of bacteriostatic water dramatically changes the concentration, which is why consistency is key

Two separately opened vials, by contrast, are exposed to repeated septum punctures, ambient temperature excursions during handling, and the cumulative oxidative load that comes with each reconstitution event