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On the other hand, in cholestatic individuals in whom the NGS panels have not found pathogenic mutations, a comprehensive study like WES, performed over the full exome, can find potential gene candidates for a cholestasis association, as was the case with the most recently discovered PFIC, PFIC 13 [37, 90]

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Researchers exploring peptide stacking approaches should understand that untested combinations carry inherently higher risk than established protocols

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