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This procedure involved removing the supernatant and resuspending the mitochondrial pellet in an isolation buffer of 250 mM sucrose, 0.5 mM EDTA, and 10 mM Tris-HCl to achieve a final protein concentration of 20-25 mg/ml [2]
High-purity peptides are commonly used to explore cellular pathways, receptor interactions, and drug bioavailability
In another study conducted on rats, the inferior vena cava vein was ligated, which commonly results in a disturbance of blood flow, vessel injury and formation of thrombus
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