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Fresh plant tissue (0.5 g) was homogenized in 5 mL of 5% trichloroacetic acid (TCA) and centrifuged at 4000 rpm for 10 min at 4C
May also be mixed with your favorite beverage
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Without biomarker-guided intervention, metabolomic research may remain descriptive even when biologically sophisticated
The 10 mL volume is well suited for laboratories conducting moderate-scale reconstitution protocols that require a reliable, preserved diluent capable of maintaining the stability and solubility of sensitive peptide compounds throughout extended experimental timelines and repeated sampling events
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