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Methods Computational analysissingle-cell sequencing datasets Table 2 summarizes the single-cell sequencing datasets used

The aqueous phase was lyophilized (0.03 mBar, 80 C, 72 h) to obtain a white solid using a Telstar LYOQuest-85 freeze dryer (Telstar, Madrid, Spain)

To minimize such unwanted events, we developed a standardized thawing procedure for frozen samples, with a small sample volume (150-200 L): thawing under strict cooling conditions (+0C to +2C) within 12-15 minutes in an ice-water bath, without agitation

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L-carnitine is by no means a magic bullet, but if youre willing to exercise regularlyespecially if you follow the mini-fast with exercise protocolit may help