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The sixth coordination site, the reactive center, is variable, being a cyano group (CN), a hydroxyl group (OH), a methyl group (CH 3 ) or a 5-deoxyadenosyl group
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By treating metabolically active adipocytes and hepatocytes with 5-amino-1-methylquinolinium, researchers can precisely inhibit intracellular NNMT activity, monitor changes in the nicotinamide pathway and NAD+ levels, and analyze effects on energy metabolism
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The mechanism involves increased NAD+ levels in white adipose tissue, which activates sirtuins and AMPK signaling to shift cellular metabolism toward fatty acid oxidation
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