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Vacuolar sulfate transporters are essential determinants controlling internal distribution of sulfate in Arabidopsis

Methods Construct modification and expression of full-length GLP-1R The optimized gene of full-length GLP-1R was cloned into the modified pFastbac1 vector at the BamHI/HindIII sites and the native signal sequence was replaced by haemagglutinin (HA) to enhance receptor expression (see primers in Supplementary Table 2), followed by a flag-tag, a 10 His-tag and a tobacco etch virus protease (TEV) site at the N-terminus

This peptide is often incorporated into formulations targeting conditions such as alopecia

DOI: 10.1007/s00424-012-1165-0 [Google Scholar] Goldstein RH, Poliks CF, Pilch PF, Smith BD, Fine A