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Li J, et al
During treatment of the PDX model of osteosarcoma, since the individuality of the mice, the tumor volume of one mouse reached 1500 mm 3 by the end of the treatment, and this mouse was not euthanized in time to obtain reliable and intuitive experimental results
Since histidine (His) in position 2 and tryptophan (Trp) in position 3 appeared to have receptor binding and functional effects 16
Cells were then rinsed thrice using serum-free culture media to eliminate any extracellular DCFH-DA, and cells were imaged via fluorescent microscopy (Ex: 488 nm, Em: 522 nm) to assess intracellular ROS levels
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