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Membranes were probed using antibodies to FoxO1 (1:2500, 18592-1AP, Proteintech, Chicago, IL, USA), SOD2 (1:6000, 66474-1lg, Proteintech, Chicago, IL, USA), NOX4 (1:600, 14347-1-AP, Proteintech, Chicago, IL, USA), and -actin (1:2000, AM10218-400, Abgent) as the primary antibodies

The combination of these two peptides engages non-overlapping cellular axes

In contrast, the ibuprofen-only group showed significant associations across all models, while the combined paracetamol plus ibuprofen group was significant in Models A and C, but not in Model B

These findings highlight the potential of TKIs in selected ESCC populations

TB-500 is a synthetic fraction of the protein Thymosin Beta-4, a protein naturally ubiquitous in both animal and human cells