For sample cleanup, 1 L of Exo-SAP-IT (ThermoFisher) was added to 2.5 L of PCR product and incubated at 37 C for 15 min followed by a 15 min incubation at 80 C to inactivate the enzyme
Overall, the study demonstrated that high-grade serous carcinoma (HGSC) ovarian cancers can be clearly divided into groups according to GPx3 expression levels, and that GPx3 is essential for the survival of HGSC ovarian cancer cells in the unique ascitic tumor microenvironment and protect cells from extracellular oxidative stress factors (36)
Funding The authors declare that no funds, grants, or financial support were received during the preparation of this manuscript
Messing B, Zarka Y, Lmman M, Iglicki F, Coffin B, Rambaud J
Carnitine does not increase the energy metabolism