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Cells were washed once with 1 ml PBS then lysed in the plate with 0.1 ml/well 2X Laemmli buffer (Bio-Rad) supplemented with 20 mM DTT
Digested samples were desalted using a C18 spin column (Pierce C18 Spin, #89873) according to manufacturer instructions prior to resuspension in EPPS buffer and CH 3 CN for TMT labeling as described above
Dialogues Clin Neurosci 2:91100 DeMaagd G, Philip A (2015) Parkinson's disease and Its management: part 1: disease entity, risk factors, pathophysiology, clinical presentation, and diagnosis
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Dense hydroxyl polyethylene glycol dendrimer targets activated glia in multiple CNS disorders
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