Key Take-Aways for Healthy GLP-1 Management 1
Extracellular flux (XF) analysis Seahorse XF analyzer (Seahorse Bioscience, Billerica, MA) was employed to simultaneously measure the flux of oxygen (oxygen consumption rate) and protons (extracellular acidification rate) of adherent cells in a microplate over time
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Because each of these factors can influence results, treatment decisions often depend on a patients full health history and weight loss goals
Subsequently, the OCR was measured following the addition of 1 M oligomycin to inhibit ATP synthesis from oxidative phosphorylation, 1.5 M carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP) to uncouple the mitochondrial membrane that stimulates respiration, and a mixture containing 0.5 M each of antimycin A and rotenone (A+R) to inhibit complex I and III that terminates mitochondrial oxidative phosphorylation