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The thermocycler conditions were 95 C for 30 s for 1 cycle, 95 C for 5 s, and 60 C for 10 s for 40 cycles

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While toxicity is rare, excessive intake of certain B vitamins (particularly B or B) from multiple sources could increase the risk of side effects (like niacin flush or peripheral neuropathy)

Patients with vitamin B12 deficiency and concurrent renal or hepatic disease may require increased doses or more frequent administration of methylcobalamin

[2] The bar for FDA drug approval is extremely high, and many compounds that fall short of that bar still have meaningful clinical utility under medical supervision