I use with an exfoliating sponge
A., Martinez-Cue, C., Garcia-Porrero, J
The female mice (homozygous for Hba tm1(HBA)Tow and heterozygous for Hbb tm2(HBG1,HBB*)Tow and Hbb tm3(HBG1,HBB)Tow [h/h:: A / S ], sickle-cell trait mice) and male mice (homozygous for both Hba tm1(HBA)Tow and Hbb tm2(HBG1,HBB*)Tow [h/h:: S / S ], sickle SCD mice) were bred to generate SCD mice
cDNAs of tissues total RNA were synthesized using a PrimerScript TM RT reagent Kit with a gDNA Eraser (Perfect Real Time)(Takara, Japan) by S1000 TM Thermal Cycler (Bio-Rad, USA)
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