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Diabetes Mellitus, Race, and Effects of Omega-3 Fatty Acids on Incidence of Heart Failure Hospitalization

doi: 10.1530/EJE-12-0807 59 SchernthanerGPragerRPunzengruberCLugerA

In brief, HCT116 cells were infected with the GeCKO v1 human CRISPR knockout pooled library using lentiviral transduction at a multiplicity of infection of 0.3, and populations stably expressing gRNAs were selected for using puromycin and expanded for 8 days to allow for essential gene drop-out

A high-sucrose, high-fat diet causes harmful oxidative modification in metabolically active proteins, resulting in maladaptive tissue remodeling and potentially exacerbating diastolic left ventricular dysfunction

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