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SDS-PAGE and Western Blot analysis Twenty L of sample containing 5 mg of purified rhGPx3 mutant was mixed with 20 L of loading buffer (20 mM Tris-HCl, 8% SDS, 10% -mercaptoethanol, 30% glycerol and 0.004% bromophenol blue at pH 6.8) and heated in boiling water for 5 min

LY3437943, a triple GCGR/GIPR/GLP-1R agonist

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Expression of mt-roGFP in park 13 tyrosine hydroxylase positive neurons showed significantly reduced mitochondrial ROS accumulation under Sp treatment as compared to untreated ones, highlighting potential mitochondrial recovery and prevention of oxidation induced cell death in dopamine producing neurons (Fig